Engineering of an intersubunit disulphide bridge in glutathione reductase fromEscherichia coli
نویسندگان
چکیده
منابع مشابه
Flavoprotein disulphide oxidoreductases: protein engineering of glutathione reductase from Escherichia coli.
Reduced glutathione (GSH) plays a crucial role in ensuring that other thiol groups remain reduced within the cell and is particularly important in the biosynthesis of DNA [for ;I review, see Holmgren ( 1985)l. Dihydrolipoamide dehydrogenase is an essential component of the 2-ox0 acid dehydrogenase multi-enzyme complexes (Reed, 1974; Perham, 1983) in which it acts to oxidize the dihydrolipoic ac...
متن کاملA glutathione reductase from Escherichia coli.
Earlier studies had demonstrated that a flavoprotein capable of mediating a triphosphopyridine nucleotide (TPN)-linked reduction of Furacin (5-nitro-2-furaldehyde semicarbazone) was present in cell-free extracts of Furacin-susceptible strains of Escherichia coli, but was absent in extracts of resistant strains (I). Dr. Philip E. Hartman, who was then associated with our department, suggested to...
متن کاملA single intersubunit salt bridge affects oligomerization and catalytic activity in a bacterial quinone reductase.
YhdA, a thermostable NADPH:FMN oxidoreductase from Bacillus subtilis, reduces quinones via a ping-pong bi-bi mechanism with a pronounced preference for NADPH. The enzyme occurs as a stable tetramer in solution. The two extended dimer surfaces are packed against each other by a 90 rotation of one dimer with respect to the other. This assembly is stabilized by the formation of four salt bridges b...
متن کاملGlutathione reductase is not required for maintenance of reduced glutathione in Escherichia coli K-12.
Seven independently isolated glutathione reductase-deficient (gor) Escherichia coli mutants were found to have an in vivo glutathione redox state that did not significantly differ from that of the parental strain, 98 to 99% reduced. Strains containing both a gor mutation and either a trxA mutation (thioredoxin deficient) or a trxB mutation (thioredoxin reductase deficient) were able to maintain...
متن کاملA protein disulphide reductase from pea seeds.
1. A closed-circuit respirometer has been devised for measurement of the respiratory exchange in mice. It is particularly suitable for 14C-tracer experiments. 2. In the post-absorptive state the New Zealand strain of obese mice has a greater oxygen consumption and carbon dioxide production/g. body weight than a control strain of normal mice. 3. New Zealand obese mice oxidize [1-14C]or [2-14C]-a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 1988
ISSN: 0014-5793
DOI: 10.1016/0014-5793(88)81028-7